Evidence for Conformational Changes in Euglena Chloroplasts
نویسندگان
چکیده
منابع مشابه
Evidence for two RNA polymerase activities in Euglena gracilis chloroplasts.
Two types of RNA polymerase activity were isolated from Euglena gracilis chloroplasts and compared. One polymerase is tightly bound to chloroplast DNA; this complex is called the transcriptionally active chromosome (TAC) (Rushlow, K. E., Orozco, E. M., Jr., Lipper, C., and Hallick, R. B. (1980) J. Biol. Chem. 255, 3786-3792). The other activity is found in a soluble extract of Euglena chloropla...
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When green cells of Euglena gracilis, strain Z, were light-grown for several months on a solid medium containing an excess of sodium acetate (1.0% instead of the normal 0.1%), some 30% of the cells were colourless. The 'acetate-bleached' organisms, isolated by plating methods and subsequently incubated in the light in a liquid medium, regained the capacity to form chlorophyll in a few days in t...
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Chloroplasts can be obtained by gentle lysis or mild shear of spheroplasts of vitamin B(12)-deficient Euglena gracilis and then purified by isopycnic sedimentation on gradients of Ludox AM or Percoll. The chloroplasts appear compact and highly refractile by phase contrast or Hoffmann contrast microscopy. Upon incubation with [(3)H]leucine or [(35)S]methionine, the chloroplasts incorporate the a...
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Isolated chloroplasts of Euglena gracilis have been shown to be capable of incorporating amino acids into protein (2). This incorporation is inhibited by actinomycin D, a fact which suggests that the chloroplasts might be capable of DNAdependent RNA synthesis. Such RNA synthesis is also indicated in a recent report (6) showing inhibition of chlorophyll synthesis by actinomycin. In conjunction w...
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Analysis by acid polyacrylamide/urea gel electrophoresis of 14 individual mitochondrial tRNAs (mt-tRNAs) from human cells has revealed a variable decrease in mobility of the aminoacylated relative to the nonacylated form, with the degree of separation of the two forms not being correlated with the mass, polar character, or charge of the amino acid. Separation of the charged and uncharged specie...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1965
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.40.2.290